4YCI

non-latent pro-bone morphogenetic protein 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	120.280, 120.280, 221.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.863 - 3.250
R-factor	0.2406
Rwork	0.240
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ycg
RMSD bond length	0.002
RMSD bond angle	0.616
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.330
High resolution limit [Å]	3.250	14.530	3.250
Rmerge	0.133	0.042	3.394
Rmeas	0.154	0.051	3.905
Total number of observations	119773
Number of reflections	29711	365	2147
<I/σ(I)>	8.36	29.11	0.53
Completeness [%]	99.5	90.8	99.7
Redundancy	4.03

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277	0.15 M zinc acetate, 0.1 M sodium cacodylate pH 5.8, 4% isopropanol, 0.15 M nondetergent sulfobetaine (NDSB-211)