4YCH
Crystal Structure of R111K:Y134F:T54V:R132Q:P39Q:R59Y mutant of human Cellular Retinoic Acid Binding ProteinII with Retinal at 1.96 Angstrom - UV irradiated Crystal for 1 hour - 2nd cycle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.488, 58.488, 100.402 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.223 - 1.960 |
R-factor | 0.2072 |
Rwork | 0.205 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g7b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.104 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.080 | 0.544 |
Number of reflections | 14868 | |
<I/σ(I)> | 39.06 | 4.38 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.8 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 277 | 12% PEG 3350, 8% Tacsimate pH 6.0 |