4YCG
Pro-bone morphogenetic protein 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.2823,0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 120.780, 120.780, 220.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.788 - 3.300 |
| R-factor | 0.2138 |
| Rwork | 0.213 |
| R-free | 0.22950 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.588 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE (2.15) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.390 |
| High resolution limit [Å] | 3.300 | 14.760 | 3.300 |
| Rmerge | 0.264 | 0.106 | 4.553 |
| Rmeas | 0.276 | 0.111 | 4.749 |
| Total number of observations | 664651 | ||
| Number of reflections | 54034 | 578 | 3987 |
| <I/σ(I)> | 7.58 | 25.15 | 0.55 |
| Completeness [%] | 99.8 | 95.2 | 100 |
| Redundancy | 23.3 | 23.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 0.15 M zinc acetate, 0.1 M sodium cacodylate pH 5.8, 4% isopropanol, 0.15 M nondetergent sulfobetaine (NDSB-211) |
