4YCG

Pro-bone morphogenetic protein 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-21
Detector	PSI PILATUS 6M
Wavelength(s)	1.2823,0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	120.780, 120.780, 220.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.788 - 3.300
R-factor	0.2138
Rwork	0.213
R-free	0.22950
Structure solution method	SIRAS
RMSD bond length	0.002
RMSD bond angle	0.588
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE (2.15)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.390
High resolution limit [Å]	3.300	14.760	3.300
Rmerge	0.264	0.106	4.553
Rmeas	0.276	0.111	4.749
Total number of observations	664651
Number of reflections	54034	578	3987
<I/σ(I)>	7.58	25.15	0.55
Completeness [%]	99.8	95.2	100
Redundancy	23.3		23.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277	0.15 M zinc acetate, 0.1 M sodium cacodylate pH 5.8, 4% isopropanol, 0.15 M nondetergent sulfobetaine (NDSB-211)