4YC5
Beta1 synthetic solenoid protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.04030 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 64.570, 64.570, 160.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.570 - 1.755 |
R-factor | 0.159 |
Rwork | 0.158 |
R-free | 0.18450 |
Structure solution method | MIR |
Starting model (for MR) | 3du1 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.981 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.570 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.099 | 0.830 |
Number of reflections | 34852 | |
<I/σ(I)> | 7.2971 | 1.01 |
Completeness [%] | 99.7 | 99.85 |
Redundancy | 13.9 | 14.24 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M Bis Tris, 3.0 M sodium chloride. |