4YC3

CDK1/CyclinB1/CKS2 Apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.87260
Spacegroup name	P 21 21 21
Unit cell lengths	69.200, 70.150, 156.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.190 - 2.700
R-factor	0.2138
Rwork	0.211
R-free	0.25950
RMSD bond length	0.022
RMSD bond angle	2.221
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.190	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.152	0.700
Number of reflections	21241
<I/σ(I)>	6.6	1.5
Completeness [%]	98.4	89.4
Redundancy	3.5	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	277	0.1M MES/imidazole buffer (pH6.7), 6.5% MPD, 5% PEG4K, 10% PEG1K Protein at 10-12 mg/ml