4YBL
Crystal structure of the stabilized inner domain of clade A/E HIV-1 gp120 in complex with the ADCC mediating ANTI-HIV-1 antibody A32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.12789 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.742, 208.209, 73.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.100 |
| R-factor | 0.2346 |
| Rwork | 0.232 |
| R-free | 0.28937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TNM and 4RQH |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.527 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.186 | 0.955 |
| Number of reflections | 19959 | |
| <I/σ(I)> | 14.6 | 1.3 |
| Completeness [%] | 93.2 | 96.8 |
| Redundancy | 8.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 18-22% PEG 6000 or 8000 0.1 M Tris-HCl pH 8.5 |
| 2 | ||||
| 3 |
