4YAP

Crystal structure of LigG-apo form from Sphingobium sp. strain SYK-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99996
Spacegroup name	P 32 2 1
Unit cell lengths	64.664, 64.664, 120.731
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.055 - 1.111
R-factor	0.1685
Rwork	0.168
R-free	0.18220
RMSD bond length	0.019
RMSD bond angle	1.837
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.110
Number of reflections	115374
<I/σ(I)>	24
Completeness [%]	99.8
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Bis-Tris propane pH 7.0, 1.5 M Ammonium Sulfate