4YAC
Crystal structure of LigO in complex with NADH from Sphingobium sp. strain SYK-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.298, 79.298, 71.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.728 - 1.699 |
| R-factor | 0.1759 |
| Rwork | 0.174 |
| R-free | 0.19940 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.728 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 29261 |
| <I/σ(I)> | 17.29 |
| Completeness [%] | 100.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Ammonium Citrate, 0.1 M MES pH 5.5, 20% PEG 3,350, 5% Isopropanol |
