4Y98

Crystal structure of LigD-apo form from Sphingobium sp. strain SYK-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	61.301, 61.301, 134.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.389 - 2.648
R-factor	0.2135
Rwork	0.211
R-free	0.25440
RMSD bond length	0.007
RMSD bond angle	0.939
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.389
High resolution limit [Å]	2.640
Number of reflections	9044
<I/σ(I)>	25.2
Completeness [%]	99.9
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M Hepes pH 7.5, 1.5 M Lithium Sulfate