4Y8A
Crystal Structure of the N-terminal domain of CEACAM6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 63 |
| Unit cell lengths | 96.660, 96.660, 54.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.710 - 1.830 |
| R-factor | 0.1688 |
| Rwork | 0.167 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qsq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.530 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.710 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 1.134 | |
| Number of reflections | 24029 | |
| <I/σ(I)> | 7.3 | 1.5 |
| Completeness [%] | 94.5 | 95.1 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 10% PEG 8000, 0.1 M Sodium Acetate, pH 4.6, 0.2 M Zinc Acetate |
