4Y89
Crystal structure of the N-terminal domain of CEACAM7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.623, 64.886, 103.164 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 103.160 - 1.470 |
| R-factor | 0.1455 |
| Rwork | 0.143 |
| R-free | 0.19340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qsq |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.901 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.160 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.444 | |
| Number of reflections | 71599 | |
| <I/σ(I)> | 28 | 1.57 |
| Completeness [%] | 98.2 | 82.7 |
| Redundancy | 2.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 18% PEG 8000, 0.1 M Tris, pH 8.5, 0.2M Magnesium Chloride |
