4Y7P

Structure of alkaline D-peptidase from Bacillus cereus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-06-14
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	104.019, 104.019, 98.535
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.220 - 2.100
R-factor	0.17609
Rwork	0.174
R-free	0.21922
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hvb
RMSD bond length	0.023
RMSD bond angle	2.119
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.068	0.487
Number of reflections	69524
<I/σ(I)>	33.3
Completeness [%]	99.9
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	20% PEG3350, 0.2M sodium thiocyanate, 3% D-glucose