4Y7N

The Structure Insight into 5-Carboxycytosine Recognition by RNA Polymerase II during Transcription Elongation.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	C 1 2 1
Unit cell lengths	168.181, 222.614, 192.829
Unit cell angles	90.00, 101.59, 90.00

Refinement procedure

Resolution	48.880 - 3.300
R-factor	0.209
Rwork	0.207
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m3y
RMSD bond length	0.009
RMSD bond angle	1.326
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.360
High resolution limit [Å]	3.300	8.940	3.300
Rmerge	0.153	0.067	0.762
Total number of observations	390896
Number of reflections	105724
<I/σ(I)>	5.7
Completeness [%]	99.4	100	96.5
Redundancy	3.7	3.8	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	390 mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50 mM dioxane, 10 mM DTT, and 10.7-11.6%(w/v) PEG6000