4Y6W

Crystal structure of Podosopora anserina putative kinesin light chain nearly identical TPR-like repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	80
Detector technology	PIXEL
Collection date	2012-10-20
Detector	PSI PILATUS 6M
Wavelength(s)	1.0375
Spacegroup name	P 21 21 2
Unit cell lengths	81.071, 92.341, 30.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.120 - 1.587
R-factor	0.1794
Rwork	0.178
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y6c
RMSD bond length	0.005
RMSD bond angle	0.951
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.120	1.643
High resolution limit [Å]	1.587	1.587
Rmerge	0.114
Number of reflections	32123
<I/σ(I)>	18.6	7
Completeness [%]	99.7	98.14
Redundancy	14.2	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295.16	0.1 M MES, 30% PEG 4000, 5mM TCEP