4Y6U
Mycobacterial protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | I 41 |
| Unit cell lengths | 99.280, 99.280, 128.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.202 - 2.271 |
| R-factor | 0.1856 |
| Rwork | 0.184 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dec |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.384 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.200 | 2.352 |
| High resolution limit [Å] | 2.271 | 2.271 |
| Rmerge | 0.061 | 0.479 |
| Number of reflections | 52965 | |
| <I/σ(I)> | 10.68 | |
| Completeness [%] | 99.3 | 98.78 |
| Redundancy | 4.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 14% PEG 8000, 0.3-0.5 M Li sulfate |
