4Y66
Crystal structure of Giardia lamblia Hop2-Mnd1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97889 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.847, 69.066, 292.137 |
| Unit cell angles | 90.00, 95.34, 90.00 |
Refinement procedure
| Resolution | 40.650 - 3.200 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.340 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 34750 | |
| <I/σ(I)> | 22.1 | 3.1 |
| Completeness [%] | 87.0 | 64.9 |
| Redundancy | 3.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 3350, Tascimate, Tris |
