4Y65

Crystal structure of E.coli CutA1 C16A/C39A/C79A mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	49.729, 62.016, 50.238
Unit cell angles	90.00, 118.47, 90.00

Refinement procedure

Resolution	50.000 - 1.700
Rwork	0.191
R-free	0.22320
RMSD bond length	0.005
RMSD bond angle	1.103
Data reduction software	SCALEPACK
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	27902
<I/σ(I)>	4.8
Completeness [%]	95.0
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M HEPES-Na pH7.5 , 1.4M tri-Sodium Citrate dihydrate