4Y5J
Drosophila melanogaster Mini spindles TOG3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97723 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 49.824, 49.824, 204.992 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.165 - 2.303 |
| R-factor | 0.1827 |
| Rwork | 0.176 |
| R-free | 0.24090 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 13396 | |
| <I/σ(I)> | 33.4 | 5.1 |
| Completeness [%] | 99.5 | 95 |
| Redundancy | 10.8 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG4000, potassium phosphate monobasic |
