4Y35
Endothiapepsin in complex with fragment 290
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.251, 73.107, 52.776 |
| Unit cell angles | 90.00, 109.53, 90.00 |
Refinement procedure
| Resolution | 39.564 - 1.457 |
| R-factor | 0.1265 |
| Rwork | 0.125 |
| R-free | 0.15870 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.211 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.8.4_1492) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.130 | 1.550 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.442 | |
| Number of reflections | 56120 | |
| <I/σ(I)> | 13.3 | 3.4 |
| Completeness [%] | 99.3 | 97.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 290 | 0.1M ammonium acetate, 0.1M sodium acetate, 24-30% PEG 4000 crystals obtained by streak-seeding |
