4Y2L
Structure of CFA/I pili major subunit CfaB trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.266, 105.811, 70.109 |
| Unit cell angles | 90.00, 114.05, 90.00 |
Refinement procedure
| Resolution | 40.783 - 1.746 |
| R-factor | 0.2044 |
| Rwork | 0.204 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f84 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.556 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1323)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.783 | 1.820 |
| High resolution limit [Å] | 1.746 | 1.750 |
| Rmerge | 0.527 | |
| Number of reflections | 85281 | |
| <I/σ(I)> | 8.3 | 1.54 |
| Completeness [%] | 96.4 | 91.6 |
| Redundancy | 3.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M NaOH-citrate, pH 5.0, 20% PEG8000 |
