4Y1K
PALMITOYLATED OPRM OUTER MEMBRANE FACTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2004-11-25 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.642, 87.858, 355.942 |
| Unit cell angles | 90.00, 98.94, 90.00 |
Refinement procedure
| Resolution | 87.910 - 3.800 |
| R-factor | 0.299 |
| Rwork | 0.297 |
| R-free | 0.34600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d5k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.405 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.000 | 4.010 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.312 | |
| Number of reflections | 40566 | |
| <I/σ(I)> | 8.5 | 3.3 |
| Completeness [%] | 87.7 | 77.9 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 100 MM SODIUM ACETATE (PH 4.5), 6% PEG 20 000 (W/V), 300 MM AMMONIUM CITRATE, 25-30% GLYCEROL (V/V) 0.9% BETAOG |
