4Y1F
SAV1875-E17D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.313, 94.150, 42.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.310 - 1.800 |
| R-factor | 0.19549 |
| Rwork | 0.194 |
| R-free | 0.22444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y0n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.330 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.068 | 0.056 | 0.163 |
| Rmeas | 0.075 | 0.063 | 0.174 |
| Rpim | 0.030 | 0.026 | 0.061 |
| Total number of observations | 221449 | ||
| Number of reflections | 30762 | ||
| <I/σ(I)> | 26.8 | ||
| Completeness [%] | 99.3 | 90.4 | 100 |
| Redundancy | 7.2 | 5.8 | 8.2 |
| CC(1/2) | 0.994 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PPG400, Bis-Tris |
