4Y0L

Mycobacterial membrane protein MmpL11D2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	65.600, 91.730, 32.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.359 - 2.400
R-factor	0.2125
Rwork	0.208
R-free	0.25370
Structure solution method	SAD
Data reduction software	iMOSFLM
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.360	53.360	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.109	0.088	0.290
Rpim	0.048	0.047	0.127
Total number of observations	23972	381	2567
Number of reflections	4128
<I/σ(I)>	20.5	44.5	7.8
Completeness [%]	99.9	94.3	100
Redundancy	5.8	4.1	6.1
CC(1/2)	0.992	0.987	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	0.1 M MES, pH 6.7, 2 M MgSO4