4Y07

Crystal structure of the HECT domain of human WWP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97923
Spacegroup name	P 41 21 2
Unit cell lengths	61.765, 61.765, 242.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.111 - 2.507
R-factor	0.2463
Rwork	0.244
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nd7
RMSD bond length	0.003
RMSD bond angle	0.648
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.507	5.600	2.507
Rmerge	0.132	0.059	0.953
Total number of observations	421734
Number of reflections	16298
<I/σ(I)>	9.9
Completeness [%]	99.9	99.3	100
Redundancy	27.4	24.2	28.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	277	0.2M MgCl2, 0.1M Hepes pH8.4 and 15% ethanol