4Y05
KIF2C short Loop2 construct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.889, 83.889, 147.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.720 - 2.590 |
| R-factor | 0.1928 |
| Rwork | 0.192 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2heh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 2.730 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmeas | 0.054 | |
| Rpim | 0.015 | 0.009 |
| Total number of observations | 234047 | 7349 |
| Number of reflections | 17174 | |
| <I/σ(I)> | 27.6 | 75.8 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 13.6 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M AMMONIUM SULFATE, 10% ETHYLENEGLYCOL, 0.1M TRIS-HCL PH8.0 |
