4XZZ
Structure of Helicobacter pylori Csd6 in the ligand-free state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.963, 91.028, 127.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.030 |
| R-factor | 0.19825 |
| Rwork | 0.196 |
| R-free | 0.24813 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.325 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.095 | 0.470 |
| Number of reflections | 47555 | |
| <I/σ(I)> | 25 | 3 |
| Completeness [%] | 98.3 | 98.1 |
| Redundancy | 4.82 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 1.5%(v/v) Tacsimate (pH 7.0), 22%(w/v) PEG 3350, 100mM sodium-HEPES |
