4XZR
Structure of yeast importin a bound to the membrane protein Nuclear Localization Signal sequence of INM protein Heh1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2013-02-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.682, 58.306, 95.372 |
| Unit cell angles | 90.00, 129.32, 90.00 |
Refinement procedure
| Resolution | 29.008 - 2.250 |
| R-factor | 0.1973 |
| Rwork | 0.196 |
| R-free | 0.21540 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.792 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1696)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 25906 |
| <I/σ(I)> | 31.4 |
| Completeness [%] | 98.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM ammonium acetate, 20% PEG 8000, 100 mM BisTris |
