4XZF

Crystal structure of HIRAN domain of human HLTF in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-07
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	147.399, 33.418, 36.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.600 - 1.380
R-factor	0.1667
Rwork	0.165
R-free	0.20668
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	2.018
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.380
Number of reflections	36316
<I/σ(I)>	17.8
Completeness [%]	94.1
Redundancy	5.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG 3350, Na acetate