4XZE

The crystal structure of Hazara virus nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	67.404, 72.170, 453.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.220 - 2.900
R-factor	0.2105
Rwork	0.206
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u3i
RMSD bond length	0.009
RMSD bond angle	0.922
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge		0.352
Number of reflections	42788
<I/σ(I)>	11.008	0.327
Completeness [%]	84.7	100
Redundancy	6	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	200mM potassium citrate tribasic monohydrate (PH 8.0), 5mM manganese chloride,14% (wt/vol) PEG 3350