4XZB
endo-glucanase GsCelA P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-05 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.957, 71.673, 92.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.620 |
| R-factor | 0.1603 |
| Rwork | 0.159 |
| R-free | 0.17824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pzt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.299 |
| Data reduction software | Coot |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.074 | 0.495 |
| Number of reflections | 35214 | |
| <I/σ(I)> | 32.4 | 4 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 16%(w/v) PEG4000, 0.2M imidazole malate |
