4XYP
Crystal structure of a piscine viral fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-14 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.979490 |
Spacegroup name | H 3 2 |
Unit cell lengths | 42.874, 42.874, 232.245 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.707 - 2.100 |
R-factor | 0.201 |
Rwork | 0.196 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.234 |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER (2.5.3) |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.710 | 38.710 | 2.160 |
High resolution limit [Å] | 2.100 | 8.920 | 2.100 |
Rmerge | 0.066 | 0.036 | 0.495 |
Rpim | 0.032 | 0.023 | 0.247 |
Total number of observations | 23796 | 325 | 1453 |
Number of reflections | 5037 | ||
<I/σ(I)> | 13.7 | 27.8 | 3.2 |
Completeness [%] | 97.6 | 93.8 | 88.6 |
Redundancy | 4.7 | 3.7 | 4 |
CC(1/2) | 0.999 | 0.999 | 0.833 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 1:1 volume ratio of 30mg/mL protein solution and 0.2 M lithium sulfate, 0.1 M sodium acetate, pH 4.5, 2 mg/mL tetramethylthionine chloride and 50% (v/v) PEG 400 |