4XXP
Crystal Structure of an Uncharacterized Protein (Rv0315 ortholog) from Mycobacterium paratuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-31 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.030, 49.530, 55.310 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 36.830 - 1.600 |
| R-factor | 0.1427 |
| Rwork | 0.140 |
| R-free | 0.17060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pq9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.157 |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 | |
| High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
| Rmerge | 0.045 | 0.037 | 0.210 |
| Rmeas | 0.046 | 0.038 | 0.225 |
| Total number of observations | 293026 | ||
| Number of reflections | 24420 | 303 | 1586 |
| <I/σ(I)> | 35.5 | 86.16 | 8.04 |
| Completeness [%] | 94.8 | 99 | 83.5 |
| Redundancy | 12 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 290 | JCSG+(c1): 20% PEG-8000, 100mM Sodium phosphate dibasic/ citric acid, pH=4.2, 200mM Sodium Chloride mixed with MypaA.18623.a.B2.PS01983 at 26.32 mg/ml, tray 252788c1258195b4 |
