4XXE
Structure of AgrA LytTR domain in complex with promoters
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 42 |
| Unit cell lengths | 96.760, 96.760, 51.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.760 - 3.200 |
| R-factor | 0.3187 |
| Rwork | 0.315 |
| R-free | 0.35517 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.436 |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.030 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 8048 | |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 6% Tacsimate pH 7.0 0.1M MES monohydrate pH 6.0 25% PEG4000 0.1M CoCl2 hexahydrate |
