4XX1
Low resolution structure of LCAT in complex with Fab1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.2499 |
| Spacegroup name | P 32 |
| Unit cell lengths | 166.419, 166.419, 97.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.062 - 3.600 |
| R-factor | 0.1998 |
| Rwork | 0.196 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xwg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.992 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 144.123 | 80.847 | 3.790 |
| High resolution limit [Å] | 3.600 | 11.380 | 3.600 |
| Rmerge | 0.105 | 0.906 | |
| Rmeas | 0.182 | ||
| Rpim | 0.078 | 0.050 | 0.451 |
| Total number of observations | 189720 | 6123 | 24982 |
| Number of reflections | 34818 | ||
| <I/σ(I)> | 7 | 14.7 | 2.1 |
| Completeness [%] | 99.4 | 99.5 | 99 |
| Redundancy | 5.4 | 5.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M di-ammonium tartrate, 0.001 M zinc acetate, 13% PEG 1500 |
