4XWU

Structure of the IMP dehydrogenase from Ashbya gossypii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	I 4
Unit cell lengths	106.787, 106.787, 69.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.390 - 1.750
R-factor	0.1683
Rwork	0.167
R-free	0.19220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4avf
RMSD bond length	0.006
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.390	1.813
High resolution limit [Å]	1.750	1.750
Rmerge	0.040	1.370
Number of reflections	39565
<I/σ(I)>	30.28	1.88
Completeness [%]	100.0	100
Redundancy	12.4	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M HEPES pH 7.5, 40% PEG-300, 0.2 M NaCl