4XWU
Structure of the IMP dehydrogenase from Ashbya gossypii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 4 |
| Unit cell lengths | 106.787, 106.787, 69.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.390 - 1.750 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4avf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.390 | 1.813 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.040 | 1.370 |
| Number of reflections | 39565 | |
| <I/σ(I)> | 30.28 | 1.88 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M HEPES pH 7.5, 40% PEG-300, 0.2 M NaCl |
