4XWJ

Histidine-containing phosphocarrier protein (HPr) and antisigma factor Rsd complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-13
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 32 2 1
Unit cell lengths	74.768, 74.768, 72.753
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.579 - 2.095
R-factor	0.222
Rwork	0.218
R-free	0.29890
RMSD bond length	0.019
RMSD bond angle	1.841
Data reduction software	d*TREK
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	13804
<I/σ(I)>	44.43
Completeness [%]	97.2
Redundancy	15.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	0.1 M NaCl, 0.1 M HEPES (pH 7.5), 1.6 M ammonium sulfate