4XW7

Crystal structure of the ZMP riboswitch at 2.50 angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	40.160, 40.160, 234.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.565 - 2.500
R-factor	0.252
Rwork	0.249
R-free	0.27350
Structure solution method	SAD
RMSD bond length	0.001
RMSD bond angle	0.421
Data reduction software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.530
High resolution limit [Å]	2.500	2.500
Rmerge	0.062	0.159
Number of reflections	7349
<I/σ(I)>	4.2	4.2
Completeness [%]	99.3	93.9
Redundancy	7.8	5.5
CC(1/2)		0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		303.15	PEG 8K, ammonium acetate, Magnesium acetate, cacodylate