4XVZ
MycF mycinamicin III 3'-O-methyltransferase in complex with Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.864, 148.587, 66.855 |
| Unit cell angles | 90.00, 120.23, 90.00 |
Refinement procedure
| Resolution | 45.600 - 2.490 |
| R-factor | 0.18014 |
| Rwork | 0.178 |
| R-free | 0.21695 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.701 |
| Data reduction software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 86.850 |
| High resolution limit [Å] | 2.490 |
| Number of reflections | 36246 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 100.0 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.1 | 20-30% PEG 3350, 100 mM BisTrisPropane, pH 6.5 |
