Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 139.104, 139.104, 253.093 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.600 - 2.300 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ws9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.250 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.118 | 0.821 |
| Number of reflections | 42053 | |
| <I/σ(I)> | 14.2 | 2.8 |
| Completeness [%] | 99.4 | 96.5 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 23 % PEG 3350, 0.2 M DIAMMONIUM HYDROGEN CITRATE, PH 5.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K |
