4XVS
Crystal structure of HIV-1 donor 45 d45-01dG5 coreE gp120 with antibody 45-VRC01.H01+07.O-863513/45-VRC01.L01+07.O-110653 (VRC07_1995)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-04-02 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.641, 69.334, 200.546 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.820 - 1.900 |
R-factor | 0.1949 |
Rwork | 0.194 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xvt |
RMSD bond length | 0.007 |
RMSD bond angle | 1.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.820 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.150 | 0.900 |
Number of reflections | 69542 | |
<I/σ(I)> | 9.5 | |
Completeness [%] | 92.8 | 88.6 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M Na Cacodylate pH 6.5, 0.2 M NaAcetate, 6 % PEG 8000 |