4XVQ

H-Ras Y137E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-12-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	39.046, 39.046, 158.737
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.493 - 1.887
R-factor	0.2075
Rwork	0.203
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctq
RMSD bond length	0.008
RMSD bond angle	1.190
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.910
High resolution limit [Å]	1.880	5.100	1.880
Rmerge	0.054	0.045	0.318
Total number of observations	58204
Number of reflections	11267
<I/σ(I)>	22.3
Completeness [%]	92.1	83.2	39.2
Redundancy	5.2	5.4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	152mM Ca(OAc)2, 24.8% PEG 3350, 4.8% stabilization buffer at pH 7.5.