4XVN
Crystal structure of the small terminase from thermophilic phage G20C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 152.585, 152.585, 108.805 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.390 - 2.600 |
| R-factor | 0.2499 |
| Rwork | 0.249 |
| R-free | 0.27620 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.857 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.082 | 0.998 |
| Number of reflections | 29032 | |
| <I/σ(I)> | 13.8 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Hepes pH 7.5, 0.4 M ammonium acetate, 18 % (w/v) PEG 3.35 K |
