4XVM
Binary complex of human polymerase nu and DNA with the finger domain closed and thumb domain rotated out
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 97.995, 110.924, 277.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.699 - 3.200 |
| R-factor | 0.2445 |
| Rwork | 0.242 |
| R-free | 0.28120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xvi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.683 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 8.620 | 3.200 |
| Rmerge | 0.098 | 0.038 | 0.619 |
| Total number of observations | 131464 | ||
| Number of reflections | 25080 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 98.9 | 95.7 | 98.6 |
| Redundancy | 5.2 | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.5 M Ammonium Sulfate, 100 mM MES |
