4XVM

Binary complex of human polymerase nu and DNA with the finger domain closed and thumb domain rotated out

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	97.995, 110.924, 277.418
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.699 - 3.200
R-factor	0.2445
Rwork	0.242
R-free	0.28120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xvi
RMSD bond length	0.013
RMSD bond angle	1.683
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.260
High resolution limit [Å]	3.200	8.620	3.200
Rmerge	0.098	0.038	0.619
Total number of observations	131464
Number of reflections	25080
<I/σ(I)>	9
Completeness [%]	98.9	95.7	98.6
Redundancy	5.2	5	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.5 M Ammonium Sulfate, 100 mM MES