4XVL

Binary complex of human polymerase nu and DNA with the finger domain open

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	292.125, 292.125, 108.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.717 - 3.301
R-factor	0.2039
Rwork	0.203
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.076
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.360
High resolution limit [Å]	3.300	8.880	3.300
Rmerge	0.132	0.032	0.696
Total number of observations	148779
Number of reflections	26307
<I/σ(I)>	7.3
Completeness [%]	99.4	98.1	98.6
Redundancy	5.7	5.5	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.5 M Ammonium Sulfate, 100 mM MES