4XVL
Binary complex of human polymerase nu and DNA with the finger domain open
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 292.125, 292.125, 108.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.717 - 3.301 |
| R-factor | 0.2039 |
| Rwork | 0.203 |
| R-free | 0.22320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.076 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 8.880 | 3.300 |
| Rmerge | 0.132 | 0.032 | 0.696 |
| Total number of observations | 148779 | ||
| Number of reflections | 26307 | ||
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.4 | 98.1 | 98.6 |
| Redundancy | 5.7 | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.5 M Ammonium Sulfate, 100 mM MES |
