4XVK

Binary complex of human polymerase nu and DNA with the finger domain closed

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	290.876, 290.876, 110.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.640 - 2.950
R-factor	0.2231
Rwork	0.222
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xvi
RMSD bond length	0.007
RMSD bond angle	1.061
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.000
High resolution limit [Å]	2.950	7.960	2.950
Rmerge	0.079	0.027	0.637
Total number of observations	175163
Number of reflections	37343
<I/σ(I)>	11.6
Completeness [%]	99.2	95.5	100
Redundancy	4.7	4.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.5 M Ammonium Sulfate, 100 mM MES