4XV0

Crystal structure of an endo-beta-1,4-xylanase (glycoside hydrolase family 10/GH10) enzyme from Trichoderma reesei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-05-22
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	41.708, 47.490, 136.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.476 - 1.970
R-factor	0.1251
Rwork	0.122
R-free	0.18510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tux
RMSD bond length	0.007
RMSD bond angle	1.113
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.037	0.057
Number of reflections	19529
<I/σ(I)>	65.43	36.08
Completeness [%]	81.8	81.8
Redundancy	5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M Bis-tris pH 6.5, 1.5 M ammonium sulphate