4XTO
Crystal structure of the light-driven sodium pump KR2 in the pentameric red form, pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.969 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 127.100, 238.980, 132.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.300 - 2.800 |
| R-factor | 0.23257 |
| Rwork | 0.230 |
| R-free | 0.28016 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xtn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.603 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 1.223 | |
| Number of reflections | 47136 | |
| <I/σ(I)> | 11 | 1.18 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | The crystals were grown using in meso crystallization technique |
