4XTL
Crystal structure of the light-driven sodium pump KR2 in the monomeric blue form, pH 4.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.969 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.180, 80.840, 232.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.400 - 1.450 |
| R-factor | 0.18878 |
| Rwork | 0.187 |
| R-free | 0.21626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ddl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.045 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.400 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 1.351 | |
| Number of reflections | 64511 | |
| <I/σ(I)> | 16.3 | 1.11 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | The crystals were grown using in meso crystallization technique |
