4XSQ
Structure of a variable lymphocyte receptor-like protein Bf66946 from Branchiostoma floridae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.250, 67.868, 118.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.790 |
| R-factor | 0.17959 |
| Rwork | 0.178 |
| R-free | 0.20226 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.252 |
| Data reduction software | HKL-2000 (v705b) |
| Data scaling software | HKL-2000 (v705b) |
| Phasing software | PHENIX (1.8.2-1309) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.114 | 0.495 |
| Number of reflections | 37098 | |
| <I/σ(I)> | 10 | 3.6 |
| Completeness [%] | 95.4 | 97.3 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 15% (w/v) polyethylene glycol 8000, 0.1 M Bis-Tris pH 6.2 |
