4XSJ

Crystal structure of the N-terminal domain of the human mitochondrial calcium uniporter fused with T4 lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	98.085, 98.085, 62.409
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.500 - 1.800
R-factor	0.13
Rwork	0.127
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lzm
RMSD bond length	0.010
RMSD bond angle	1.334
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Number of reflections	32604
<I/σ(I)>	15.9
Completeness [%]	97.9
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20% PEG 3350, 5% glycerol, 0.3 M ammonium sulphate and 0.1 M Bis-Tris-HCl (pH 5.5)